Ultramorphological Characteristics and Development Time of Immature Stages of Piophila casei (Diptera: Piophilidae)

Authors

  • Karine Brenda Barros-Cordeiro Universidade de Brasília
  • Welinton Ribamar Lopes Universidade de Goiás
  • Sônia Nair Báo Universidade de Brasília

DOI:

https://doi.org/10.12741/ebrasilis.v11i3.808

Keywords:

Cheese Skipper Fly, Forensic Entomology, Larvae, Medical-Veterinary, Morphology, Entomologia Forense, Larva, Medico-Veterinário, Morfologia, Mosca do Queijo

Abstract

Abstract. Piophila casei (Linneus), known as the cheese skipper fly, is a sarcosaprophagous dipteran, meaning it has a wide range of feeding habits: proteins, decaying animal and vegetable matter, and feces. The biological aspects of P. casei are relevant to several fields, including forensic entomology and human and veterinary medicine. This work presents the ultrastructure characteristics of P. casei, compares the structures present during its development using light microscopy and Scanning Electron Microscopy (SEM) and reports the development time of eggs, larvae and pupae under controlled laboratory conditions (23°C, 60% relative humidity and 12 h of light). Colony of adults previously maintained in the laboratory (third generation) was used in this study. The morphology of the insect and of structures such as the cephalopharyngeal skeleton, the anterior and posterior spiracles and the structures and organs of the head, thorax and abdomen of the first, second and third instar larvae were characterized. The total development time of the egg from laying until the emergence of the adult was 448 hours (18.66 days); this included 8 hours for embrionary development, 248 hours for larval development and 192 hours for pupal development.

Ultraestrutura e tempo de desenvolvimento de estágios imaturos de Piophila casei (Linneus)

Resumo. Piophila casei (Linneus), conhecida como “cheese skipper fly”, é uma mosca sarcosaprófaga, possui habito alimentar diverso. Pode alimentar-se de proteínas diversas, matéria animal e vegetal em decomposição e fezes. Os aspectos biológicos de P. casei são relevantes principalmente para a entomologia forense, medicina humana e veterinária. Este trabalho apresenta características da ultraestrutura de P. casei, comparando as estruturas dos três instares larvais do desenvolvimento, utilizando microscopias de luz e eletrônica de varredura (MEV), além de descrever o tempo de desenvolvimento de ovos, larvas e pupa sob condições controladas de laboratório (23 °C, 60% humidade relativa e 12 h de luz). A morfologia geral do inseto e estruturas como o esqueleto cefalofaríngeo, os espiráculos anterior e posterior,  as estruturas e órgãos da cabeça, tórax e abdômen da larva de primeiro, segundo e terceiro instares foram caracterizadas. O tempo total de desenvolvimento de ovo até a emergência do adulto foi de 448 horas (18,66 dias), sendo 8 horas para desenvolvimento embrionário, 248 horas para desenvolvimento larval e 192 horas para desenvolvimento pupal.

References

Barros-Cordeiro K.B. & J.R. Pujol-Luz, 2010. Morfologia e dura

Belcari, A. & R. Antonelli, 1992. Limiti termici ed optimun di sviluppo degli stadi preimmaginali di Piophila casei (L.), (Diptera Piophilidae), p. 107-117. In: Atti 5 Simposio sulla difesa antiparassitaria nelle industrie alimentari e la protezione degli alimenti, Piacenza, Italy.

Brindle, A., 1965. Taxonomic notes on the larvae of British Diptera No. 21-The Piophilidae. Entomology 78:158-160

Brown, B.V., A. Borkent, J.M. Cumming, D.M. Wood, N.E. Woodley & M.A. Zumbado, 2010. Manual of Central American Diptera, 2:1031-1039.

Busvine, J.R., 1980. Houseflies and blowflies. Insect and hygiene. The biology and control of insect pests of medical and domestic importance. Chapman & Hall, London, 211 p.

Candida, V.M. & R.R. Agatino, 2004. Piophila casei L. (Diptera: Piophilidae) monitoring in Cheese Ripening Storehouses. IOBC/WPRS Bulletin Journal, 27, 109-114.

Cortinhas, L.B., P.M. Mendon

Carri

Costa, P.R.P., R. Gurvitz, G. Muller & P.B. Ribeiro, 1986. Ciclo vital de Piophila casei L. (Diptera: Piophilidae). Arquivos Brasileiros Medicina Veterinaria e Zoologia 38: 191-196.

Dekeirsschieter, J., C. Frederickx, F.J. Verheggen, P. Boxho & E. Haubruge. 2013. Forensic Entomology Investigations from Doctor Marcel Leclercq (1924

Greenberg, B., 1971. Flies and disease, ecology, classification and biotic associations. Vol. 1. Princeton University Press, New Jersey, 856 p.

Grzywacz, A., T. Pape & K. Szpila, 2012. Larval morphology of the lesser housefly, Fannia canicularis. Medical and Veterinary Entomology, 26: 70-82. https://doi.org/10.1111/j.1365-2915.2011.00968.x.

Haines, C.P. & D.P. Ress, 1989. A field guide to the types of insects and mites infesting cured fish. FAO fisheries, Rome, Italy. Available on: <http://www.fao.org/docrep/003/t0146e/T0146E00.HTM>.

Hegazi, E.M., F.H. El-Gayar, I.A. Rawah & S.A. Ali, 1978. Factors affecting the bionomics of Piophila casei (L.). Zeitschrift f

Hennig, W., 1943. Piophilidae, p. 1-52. In: Lindner, E. (ed.) Die Fliegen der Palaearktischen Region, vol 5. Schweizerbart, Stuttgart.

Imms, A.D., 1957. A general textbook of entomology. 9a Ed. London, Methuen and CO LTD.

Kitching, R.L., 1976. On the protoracic spiracles of the first instar larvae of calyptrate Cyclorrhapha (Diptera). Journal of Australia Entomology Society, 15: 233-235. DOI: https://doi.org/10.1111/j.1440-6055.1976.tb01698.x.

Mart

Mart

Mart

McAlpine, J.F., V.B. Peterson, G.E. Shewell, J.H. Teskey, J.R. Vockeroth & D.N. Wood, 1981. Manual of Neartic Diptera. Research Branch Agriculture Canada, Monograph, 674 p.

Miokovic, B., J. Zivkovic & L. Kozacinski, 1997. Authochthonous Croatian

Oliveira-Costa, J. & M.M.C. Queiroz, 2008. Bionomia de D

Ottogalli, G., 2001. Atlante dei formaggi. Hoepli, Milano, Italy, 462 p.

Ozerov, A.L. & A.L. Norrbom, 2010. Piophilidae, p. 865-869. In: Brown, B.V., A. Borkent, J.M. Cumming, D.M. Wood, N.E. Woodley & M. Zumbado (Eds). Manual of Central American Diptera. Ottawa, Canada: National Research Council Press.

Pa

Papavero, N. & T.J. Pimentel, 2002. Insecta-Diptera-Piphilidae (Incl. Thyreophoridae). Fauna Amaz

Pape, T., V. Blagoderov & B.M Mostovski, 2001. Order Diptera Linnaeus, 1758. Zootaxa 3148: 222-229.

Passos, M.R.L., N.A. Barreto, R.Q. Varella, G.H.S. Rodrigues & D.A. Lewis, 2004. Penile myasis: a case report. Sexually Transmitted Infections, 80: 183-184.

Perez, I.C., 1971. Dipterous and Coleopterous pseudoparasites of the human intestine. Graellsia, 2: 161-176.

Peterson, A., 1960. Larvae of Insects: an introduction to nearctic species, Part II-Coleoptera, Dispetera, Neuroptera, Siphonaptera, Mecoptera, Trichoptera. Bookseller Image. Published by Edwards Brothers. Columbus, Ohio. Michingan, U.S.A., 395 p.

Prado e Castro, C., E. Cunha, A. Serrano & M.D. Garc

Russo, A., G.E. Cocuzza, M.C. Vasta, M. Simola & G. Virone, 2006. Life fertility tables of Piophila casei L. (Diptera: Piophilidae) reared at five different temperatures. Environmental Entomology, 35: 194-200. DOI: https://doi.org/10.1016/j.forsciint.2011.07.009.

Sacchi, L., A. Gigolo & G. Cestari, 1971. Un metodo di allevamento di Piophila casei L. Rivista di Parassitologia, 32: 289-301.

Saleh, M.S. & M.M. Sibae, 1993. Urino-genital myiasis due to P. casei. Journal Egypt Society of Parasitology, 23: 737-739.

Smith, K.G.V., 1986. A manual of forensic entomology. British Museum (Natural History) and Cornell University Press, London, 205 p.

Simmons, P., 1927. The cheese skipper as a pest in cured meats. U.S. Department of Agriculture Bulletin, 1453: 1-55.

Skevington, J.H. & P.T. Dang, 2002. Exploring the diversity of flies (Diptera). Biodivesity, 3: 3-27. DOI: https://doi.org/10.1080/14888386.2002.9712613.

Sukontason, K.L., K. Sukontason, S. Piangjai, W. Choochote, R.C. Vogtsberger & J.K. Olson, 2001. Scanning electron microscopy of the third-instar Piophila casei (Diptera: Piophilidae), a fly species of forensic importance. Journal of Medical Entomology, 38, 756-759. DOI: https://doi.org/10.1603/0022-2585-38.5.756.

Sukontason, K.L., R,C. Vogtsberger, N. Boonchu, T. Chaiwong, D. Sripakdee, R. NgernKlun, S. Piangjai & K. Sukontason, 2005. Larval morphology of Chrysomya nigripes (Diptera: Calliphoridae), a fly species of forensic importance. Journal of Medical Entomology, 42: 233-240. DOI: https://doi.org/10.1603/0022-2585(2005)042[0233:lmocnd]2.0.co;2.

Wendt, L.D., 2017. Piophilidae in Cat

Zumpt, F. 1965. Myiasis in man and animals in the old world. London, Butterworths, 267 p.

Zuska, J. & P. Lestovka, 1965. A review of the Czechoslovak species of the Piophilidae with special references to their importance to food industry (Diptera, Acaliptrata). Acta Entomology Bohemoslovaca, 62: 141-157.

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Published

2018-12-21

How to Cite

[1]
Barros-Cordeiro, K.B., Lopes, W.R. and Báo, S.N. 2018. Ultramorphological Characteristics and Development Time of Immature Stages of Piophila casei (Diptera: Piophilidae). EntomoBrasilis. 11, 3 (Dec. 2018), 201–208. DOI:https://doi.org/10.12741/ebrasilis.v11i3.808.

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Section

Morphology and Physiology